Figure 6.

TEMPOL produces significant decreases in colony size but not colony number. 4T1 cells were plated at low cell number and treated the following day with TEMPOL at varying concentrations, and the control was DMEM alone. After 5 days, cells were fixed, stained, and imaged using Alpha Imager software. (a) Colony number was counted and (b) total colony size was measured using Alpha Imager software. Data shown suggests that TEMPOL alone inhibits cellular proliferation at concentrations ranging from 18 to 180 μM, according to a two-tailed t test, with p <0.001, but is not significantly cytotoxic at the concentrations shown.