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Bivalent expression of S1PT antigen in recombinant BCG. Western blot of rBCG strains expressing S1PT through distinct vectors. Protein extracts of wild-type BCG (BCG wt), rBCG-S1PT, rBCG-S1i and rBCG-S1+S1i were separated in soluble and insoluble fractions. S1PT antigen was detected using anti-S1PT previously generated in mice. The values in the center represent the molecular sizes in kilodaltons. Arrows indicate the presence of the two forms of S1PT expressed in rBCG.
