Figure 4.

Single integrative and bivalent rBCG vaccines induce inflammatory cytokines. Splenocytes from immunized mice were cultured for 48 h in presence of CFP or medium only (mock). Cytokine levels were measured by ELISA. Data are shown as the mean (±SEM) from n = 4-5 mice per group. Statistical analyses were performed by Student's t test with a Mann-Whitney post-test. ^∗p < 0.05, ^∗∗p < 0.01; ns = not significant.