Table 2.
Ability of sRNase L KO-PK and PK-15 cells for PRV propagation.
| PRV strain | Harvest time | Virus titers in sRNase L KO-PK cells a | Virus titers in PK-15 cells a | Fold-difference in PRV titers b |
|---|---|---|---|---|
| SD1404 | 12 h | 1×10 0.6±0.1 TCID50/mL | 1×10 0.7±0.2 TCID50/mL | 0.79 |
| Bartha-K61 | 12 h | 1×10 0.6±0.2 TCID50/mL | 1×10 0.7±0.3 TCID50/mL | 0.79 |
| SD1404 | 24 h | 1×10 3.2±0.1 TCID50/mL | 1×10 3.0±0.3 TCID50/mL | 1.58 |
| Bartha-K61 | 24 h | 1×10 3.1±0.3 TCID50/mL | 1×10 2.9±0.1 TCID50/mL | 1.58 |
| SD1404 | 36 h | 1×10 5.6±0.2 TCID50/mL∗ | 1×10 4.9±0.2 TCID50/mL | 5.01 |
| Bartha-K61 | 36 h | 1×10 5.1±0.1 TCID50/mL∗ | 1×10 4.4±0.2 TCID50/mL | 5.01 |
| SD1404 | 48 h | 1×10 6.9±0.2 TCID50/mL∗ | 1×10 6.2±0.1 TCID50/mL | 5.01 |
| Bartha-K61 | 48 h | 1×10 6.4±0.2 TCID50/mL∗ | 1×10 5.6±0.3 TCID50/mL | 6.31 |
| SD1404 | 60 h | 1×10 5.9±0.3 TCID50/mL | 1×10 5.4±0.1 TCID50/mL | 3.16 |
| Bartha-K61 | 60 h | 1×10 5.3±0.1 TCID50/mL | 1×10 5.1±0.2 TCID50/mL | 1.58 |
aData represent the means ± standard deviations of three independent experiments. ∗, P < 0.05.
bFold-difference in PRV titers was obtained by PRV titers in sRNase L KO-PK cells/PRV titers in PK-15 cells.