Use of Chemical Probes to Detect Mitochondrial ROS by Flow Cytometry and Spectrofluorometry

. Author manuscript; available in PMC: 2019 Mar 17.

Published in final edited form as: Methods Enzymol. 2014;542:223–241. doi: 10.1016/B978-0-12-416618-9.00012-1

Solution A
NH₄Cl	131.08 mM
KCl	4.96 mM
Na₂HPO₄· 12H₂O	0.85 mM
KH₂PO₄	176.00 μM
Glucose	5.55 mM
Phenol red	28.22 μM
Dissolve in 1 L of distilled water and autoclave

Solution B
MgCl₂·6H₂O	1.03 mM
MgSO₄·6H₂O	0.29 mM
CaCl₂	1.16 mM
Dissolve in 0.5 L of distilled water and autoclave

Solution C
NaHCO₃	13.39 mM
Dissolve in 0.5 L of distilled water and autoclave