(A) Diagram showing positions of targets of two MafB gRNAs within the MafB gene. (B-M) In situ hybridization for Sox10 (B-D), MafB (E-G), Krox20 (H-J) or Ets1 (K-M) of HH12 embryos unilaterally electroporated with control MO (B,E,H,K) or MafB MO (C,F,I,L), or bilaterally electroporated with control and MafB gRNAs (D,G,J,M) showing reduced expression after loss of MafB. White, gray and black arrowheads point to wild-type, control and MafB-depleted cardiac neural crest streams, respectively.