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An example of blood vessel density calculation by imaging analysis software. A: Hematoxylin and eosin staining of a cancer lesion (case 43, original magnification × 100, scale bar 100 μm). B: CD31 immunohistochemistry highlights blood vessels. C: A depth of 350 μm was trimmed manually. Automatically recognized blood vessels are colored in the cancer lesion (D) and non-cancer area (E). The calculated blood vessel area and ratio in this case are shown in the table.
