Table 1.
Sequences of primers for quantitative real-time PCR
Name | Sequence (5'-3') |
Primers used for mRNA detection | |
miR-596 (forward) | AAGCCTGCCCGGCTCCT |
miR-596 (reverse) | GCTGTCAACGATACGCTACGT |
miR-596 (RT) | (GCTGTCAACGATACGCTACGTAACGGCATGACA; GTGTTTTTTTTTTTTTTTTTTTTTTTTC) |
U6 (forward) | CGCTTCGGCAGCACATATAC |
U6 (reverse) | TTCACGAATTTGCGTG TCAT |
U6 (RT) | (GCTGTCAACGATACGCTACGTAACGGCATGACAG; TGTTTTTTTTTTTTTTTTTTTTTTTTG) |
PRDX1 (forward) | AAGAAACTCAACTGCCAAGTG |
PRDX1 (reverse) | CAGCCTTTAAGACCCCATAAT |
GAPDH (forward) | CGGATTTGGTCGTATTGGG |
GAPDH (reverse) | CTGGAAGATGGTGATGGGATT |
PRDX1: Peroxiredoxin 1; MiR-596: MicroRNA-596; GAPDH: Glyceraldehyde phosphate dehydrogenase.