Figure 3.

Neuronal activity causes alkaline transients and a drop in intracellular glucose in astrocytes from wild-type but not NBCe1-KO mice. (a–d) Effect of Schaffer collateral stimulation (20 Hz, 30 s) on intracellular pH in wild-type and NBCe1-KO organotypic hippocampal astrocytes loaded with BCECF-AM. (e–j) Effect of Schaffer collateral stimulation (20 Hz, 30 s) on intracellular glucose in wild-type and NBCe1-KO hippocampal astrocytes perfused with HCO₃⁻/CO₂ saline containing 2 mM glucose and 1 mM lactate in the presence or absence of 1 µM TTX. All traces represent individual astrocytes. Bar graphs summarise the percentage of change of the BCECF-AM ratio or percentage of FRET change. The number of experiments is represented as n° of cells/ n° slices/ n° animals.