Figure 1.

Expression profile of GC and PC associated genes in sorted IgE⁺ and IgG1⁺ cell populations. (A) IL-4 and anti-CD40 stimulated tonsil B cells were harvested on day 10 of the culture and surface stained for CD138, intracellular IgE and IgG1 and FACS sorted into GC B cells (IgE^lo CD138⁻ and IgG1^lo CD138⁻), PBs (IgE^hi CD138⁻ and IgG1^hi CD138⁻), and PCs (IgE^hi CD138⁺ and IgG1^hi CD138⁺). (B) Heatmap of GC and PC associated genes in each of the sorted IgE⁺ and IgG1⁺ cell populations. Each column represents the gene expression profiles of the different phenotypic cell populations sorted from four different tonsil B cell cultures. (C) Flow cytometric validation of seven differentially expressed genes in IgE⁺ and IgG1⁺ cell populations. Data are representative of 6 experiments.