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. 2019 Mar 11;10:339. doi: 10.3389/fmicb.2019.00339

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Copyright © 2019 Bhawini, Pandey, Dubey, Zehra, Nath and Mishra.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of the relP transcription start site (TSS) by 5′ RACE. (A) Electropherogram, showing TSS (boxed nucleotide), is representative of results from sequencing of several distinct clones obtained after 5′ RACE experiments. (B) Schematic representation of S. aureus relP chromosomal region. Large and filled arrows represent the relative size, location, and transcriptional orientation of ORFs. Small and thin arrows represent the regions used to design primers for 5′RACE experiment; relP-SP1:R (1), relP-SP2:R (2), and relP-SP3:R (3). Nucleotide sequences from −78 to +44 of relP start codon (underlined) showing TSS (indicated as +1) and possible −35 and −10 elements (gray background). relP ORF is indicated by bold nucleotides. The earlier predicted TSS is shown by asterisk.