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. 2019 Mar 11;10:339. doi: 10.3389/fmicb.2019.00339

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Copyright © 2019 Bhawini, Pandey, Dubey, Zehra, Nath and Mishra.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of the transcription start site (TSS) of relQ by 5′ RACE. (A) Electropherogram showing TSS (boxed nucleotide) is representative of results from sequencing of several distinct clones obtained after 5′ RACE experiment. (B) Schematic representation of S. aureus relQ chromosomal region. Large and filled arrows represent the relative size, location, and transcriptional orientation of ORFs. Small and thin arrows represent the regions used to design primers for the 5′RACE experiment; relQ-SP1:R (1), relQ-SP2:R (2), and relQ-SP3:R (3). Nucleotide sequences from −436 to +19 of relQ start codon (underlined) showing TSS (indicated as +1) and possible −35 and −10 elements (gray background). SAUSA300_0906 ORFs are shown by italicized nucleotides, and the portion of relQ ORF is shown in bold. The earlier predicted TSS is shown with an asterisk.