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. 2019 Mar 11;10:418. doi: 10.3389/fmicb.2019.00418

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Copyright © 2019 Ma, Chen, Guan, Chen and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sensitivity of the RT-LAMP assay. Panels A–C show the turbidity in the RT-LAMP sensitivity tests for pha-NiV-BN, pha-NiV-MN, and pha-NiV-TN, respectively. LAMP-50 ng/μl of each panel represents the positive controls: pUC57-NiV-BN (50 ng/μl), pUC57-NiV-MN (50 ng/μl), and pUC57-NiV-TN (50 ng/μl), respectively. Negative control (RNA from the cell extract transfected with pHAGE-CMV-MCS-IZsGreen vector) in each panel is marked with NC-CMV and the concentrations tested in each RT-LAMP reaction are listed on the right of each panel. Panels D–F show the chromogenic test results for the RT-LAMP detection of pha-NiV-BN, pha-NiV-MN, and pha-NiV-TN, respectively, with 1 μl of calcein indicator. Numbers on the tubes of each panel correspond to the legends in panels A–C, respectively. Positive results are marked “+” and negative results are marked “−.”