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. 2019 Mar 11;12:58. doi: 10.3389/fnmol.2019.00058

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Copyright © 2019 Zhou, Chu, Xin, Li, Bai, Qiu, Yuan, Liu, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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L-Cys attenuates microglia-mediated neurotoxicity via AMPK activation. (A) Representative photographs of double immunofluorescent staining of active caspase-3 (red) and NeuN (green) within the ipsilateral cortex. Scale bar = 50 μm. Quantification of active caspase-3/NeuN-positive cells. N = 3 mice/group. Six images (20×) were randomly selected from each section per animal. (B) Effects of different conditioned media prepared from microglia-like cell line-BV2 cells on the survival of neurons as determined with TUNEL staining (red). Scale bar = 20 μm. N = 4 wells/group. Values represent the mean ± SD, ^∗∗∗p < 0.001 HI vs. Sham; ^##p < 0.01, ^###p < 0.001 HI + L-Cys vs. HI; ^&&p < 0.01 HI + L-Cys + CC vs. HI + L-Cys.