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. 2019 Mar 11;10:396. doi: 10.3389/fimmu.2019.00396

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Copyright © 2019 Rodríguez-Gómez, Talker, Käser, Stadler, Reiter, Ladinig, Milburn, Hammer, Mair, Saalmüller and Gerner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of T-bet, Eomes and GATA-3 in γδ thymocytes. (A) Following exclusion of dead cells (not shown), total TCR-γδ⁺ thymocytes were gated (top row) and analyzed for the expression of T-bet, Eomes and GATA-3 (histograms, second row). Total TCR-γδ⁺ thymocytes and TF-expressing TCR-γδ⁺ thymocytes were further analyzed for expression of CD2 and CD8α (third row, zebra plots denoted “a,” “b,” “c,” “d”). Numbers indicate the percentage of transcription factor expressing γδ thymocytes (histograms) or percentage of CD2/CD8α-defined subsets (zebra plots, third row). Data from one representative animal out of five are shown. (B) The scatter diagram shows the frequency of T-bet, Eomes and GATA-3 expressing γδ thymocytes. Each symbol represents data of one individual animal (n = 5). Green bars indicate mean values.