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. 2019 Mar 11;10:396. doi: 10.3389/fimmu.2019.00396

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Copyright © 2019 Rodríguez-Gómez, Talker, Käser, Stadler, Reiter, Ladinig, Milburn, Hammer, Mair, Saalmüller and Gerner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-4 expression in T-cell subsets after PMA/ionomycin stimulation. PBMCs isolated from the blood of 7-month-old pigs were stimulated for 4 h with PMA/ionomycin and analyzed for the expression of IL-4 in total T-cells (CD3⁺) as well as in CD4⁺ and TCR-γδ⁺ T cells. Zebra plots show live peripheral blood lymphocytes (PBL) and their expression of IL-4 vs. CD3, CD4, or TCR-γδ. CD3⁺IL-4⁺ cells, CD4⁺IL-4⁺ cells and TCR-γδ⁺IL-4⁺ cells were gated as indicated by the squares within the respective zebra plots, numbers in gates show the percentage of cells with the respective phenotype within live lymphocytes. The scatter diagram shows the percentage of CD3⁺IL-4⁺ cells, CD4⁺IL-4⁺ cells and TCR-γδ⁺IL-4⁺ cells within live PBL. Data of five individual animals (each represented by a different symbol) are shown. Green bars indicate mean values.