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. 2019 Mar 11;10:396. doi: 10.3389/fimmu.2019.00396

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Copyright © 2019 Rodríguez-Gómez, Talker, Käser, Stadler, Reiter, Ladinig, Milburn, Hammer, Mair, Saalmüller and Gerner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Frequency and phenotype of T-bet⁺ γδ T cells in different organs. (A) Following exclusion of dead cells (not shown), TCR-γδ⁺ T cells were gated within live lymphocytes (zebra plot) and further subgated to analyze the expression of T-bet. The scatter diagram shows the frequency of T-bet⁺ γδ T cells isolated from blood (peripheral blood lymphocytes, PBL), spleen (Spl), mediastinal lymph node (mLN), and lung tissue (Lg). Each symbol represents data from one individual animal (n = 8). Green bars indicate mean values. (B) γδ T cells were gated for CD2⁺ and CD2⁻ subsets and further analyzed for co-expression of perforin and T-bet. Perforin⁺ and perforin⁻ T-bet⁺ γδ T cells were then gated and investigated for expression of CD27 and CD8α. Exemplary data of the four investigated locations, blood (peripheral blood lymphocytes, PBL), spleen (Spl), mediastinal lymph node (mLN) and lung tissue (Lg) from one animal are shown. Numbers located in gates and quadrants indicate percentage of cells for one particular phenotype. For T-bet/perforin-defined subpopulations below 10%, CD8α/CD27 expression is shown in dot plots, for T-bet/perforin-defined subpopulations below 2%, CD8α/CD27 expression is not shown.