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. 2019 Mar 11;10:396. doi: 10.3389/fimmu.2019.00396

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Copyright © 2019 Rodríguez-Gómez, Talker, Käser, Stadler, Reiter, Ladinig, Milburn, Hammer, Mair, Saalmüller and Gerner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Influence of aging on the frequency of CD2⁻ and CD2⁺ γδ T cells in blood. (A) Live lymphocytes isolated from pigs at 3 weeks, 25 weeks, 26 months, and 4 years of age were analyzed for the expression of TCR-γδ vs. CD2 (zebra plots). Representative data from one animal per time point are shown. Total γδ T cells were gated and further subgated into CD2⁻ and CD2⁺ γδ T cells (not shown). (B) Stacked bar graphs indicate the frequency of CD2⁻ cells (dark gray bar) and CD2⁺ cells (light gray bar) within total γδ T cells. Data of the same five pigs are shown from 3 weeks to 26 months of age (these animals were bled repeatedly), whereas for 4 years of age, data from four different animals are presented. (C) Median fluorescence intensity (MFI) of TCR-γδ expression in CD2⁻ and CD2⁺ γδ T cells at different stages of life. Each symbol represents data from one individual animal (for 3 weeks, 25 weeks, and 26 months of age animals (n = 5) were bled repeatedly; for 4 years of age, blood was obtained from different animals (n = 4); change in animals is indicated by a dashed line). Green bars indicate mean values.