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Post-translational modification of histones in NETs. (A) Summary of the post-translational modifications to the N-terminal tails of histones H1, H2A, H2B, H3, and H4 in RA and SLE neutrophil NETs (C, citrullination; A, acetylation; M, methylation; D, dimethylation). (B) Representative immunofluorescent staining of DNA (blue), citrullinated histone H3 (green) and PADI4 (red) in PMA- and A23187-induced NETs. Images taken on an Epifluorescent microscope (Zeiss).
