FIGURE 2.

Molecular markers mapped on linkage group 4 and found associated to the male sterility locus (ms1) of C. intybus. (A) Linkage group 4 constructed from Cadalen et al. (2010). (B) Genetic distances, expressed in cM, among three SSR markers (in red), one CAPS marker derived from the MADS-box region (in blue) and the male-sterility locus, whose estimates were calculated using a BC₁ population (198 samples) segregating 1:1 for the male-sterility trait. The SSR markers were retrieved from Ghedina et al. (2015) while the CAPS marker was developed in the present work. (C) Linkage group 9 [corresponding to linkage group 4 of Cadalen et al. (2010)] constructed using the data from the Genotyping-By-Sequencing (GBS) approach and based on 44 samples of the BC₁ population mentioned above. In red are reported the two SSR already used for the first SSR-approach, in bold are listed the 13 SNP showing ≤3 recombinants with the ms1 locus. (D) Chromosomal region around the ms1, considering the recombinant data of the aforesaid 13 SNP and a total number of 108 BC₁ samples.