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. 2019 Mar 18;11(2):12. doi: 10.1038/s41368-019-0046-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Dlx2 overexpression in BMSCs has no impact on Runx2, Dlx5, Msx2 and Osxexpression. RT-qPCR analysis was performed to evaluate the expression levels of Dlx2 (a), Runx2 (b), Dlx5 (c), Msx2 (d), Osx (e), OCN (F), and Alp (g) in BMSCs transduced with Lenti-DLX2 OE (over) or Lenti-CTRL (control) at 14 and 21 days after osteogenic induction. Gapdh was used as an internal control. Statistical significance was determined as described in Fig. 1. h RT-qPCR analysis was used to evaluate the expression level of OCN and Alp upon forced overexpression of Dlx2 in MC3T3-E1 cells. i Western blot analysis was performed to measure the protein levels of OCN upon forced overexpression of Dlx2 in MC3T3-E1. β-Actin was used as an internal control. Over, BMSCs transduced with Lenti-DLX2 OE; control, BMSCs transduced with Lenti-CTRL