Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-paxillin | BD Biosciences | 610051, RRID:AB_397463 |
| Mouse anti-vinculin | Sigma-Aldrich | V9131, RRID:AB_477629 |
| Rabbit anti-non-muscle myosin IIA | Biolegend | 909801, RRID:AB_2565100 |
| Rabbit anti-non-muscle myosin IIB | Biolegend | 909901, RRID:AB_2565101 |
| Mouse anti-cardiac myosin heavy chain | Developmental Studies hybridoma bank | MF-20, RRID:AB_2147781 |
| Rabbit anti-cardiac myosin heavy chain α | Proteintech | 22281–1-AP |
| Rabbit anti-cardiac myosin heavy chain β | Proteintech | 22280–1-AP |
| Rabbit anti-beta Tubulin | Abcam | ab6046, RRID:AB_2210370 |
| Rabbit anti-actinin2 (c-terminal) | Fitzgerald | 70R-1068, RRID:AB_10809287 |
| Mouse anti-actinin2 | Abcam | ab9465, RRID:AB_307264 |
| Alexa 488-goat anti Mouse IgG | Thermo Fisher | R37120, RRID:AB_2556548 |
| Alexa 647-goat anti Rabbit IgG | Thermo Fisher | A27040, RRID:AB_2536101 |
| HRP-donkey anti Mouse IgG | Fitzgerald | 43-GM30, RRID:AB_1287764 |
| HRP-donkey anti Rabbit IgG | Fitzgerald | 43C-CB1134, RRID:AB_1287213 |
| Bacterial and Virus Strains | ||
| Stbl3 Competent Cells | Thermo Fisher | C737303 |
| Biological Samples | ||
| Cryopreserved T-cells | Hinson et.al Science, 2015 | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Blebbistatin | Calbiochem | 203390, CAS# 856925–71–8 |
| CHIR99021 | Tocris | 4423, CAS# 25291706–9 |
| IWP4 | Tocris | 5214, CAS#686772–17–8 |
| DL-lactate | Sigma | L7900, CAS# 72–173 |
| DAPI | Sigma | 10236276001, CAS#28718–90–3 |
| Fibronectin | BD Biosciences | 356009 |
| 40% acrylamide solution | BIO-RAD | 1610140 |
| 2% Bis Solution | BIO-RAD | 1610143 |
| N,N,N′,N′-Tetramethylethylenediamine | Sigma-Aldrich | T9281, CAS#110–18–9 |
| Ammonium persulfate | Sigma-Aldrich | A3678, CAS#7727–54–0 |
| NHS (N-hydroxysuccinimide) | Thermo Fisher | 24500 |
| EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) | Thermo Fisher | 22980, CAS#25952–53–8 |
| Fluospheres Carboxylate-Modified Microspheres, 0.2 μm | Thermo Fisher | F8807 |
| Alexa 488-phalloidin | Thermo Fisher | A12379 |
| Alexa 568-phalloidin | Thermo Fisher | A12380 |
| Alexa 568-NHS Ester | Thermo Fisher | A2003 |
| Pierce™ ECL Western Blotting Substrate | Thermo Fisher | 32106 |
| Pluronic F127 | Sigma | P2443, CAS# 9003–11–6 |
| SU8 photoresist | Thermo Fisher | NC0060520 |
| Experimental Models: Cell Lines | ||
| Human Induced pluripotent stem cells (hIPSC) | Hinson et.al Science, 2015 | N/A |
| hIPSC MYH7−/− | This paper | N/A |
| hIPSC MYH6−/− | This paper | N/A |
| HEK 293T | Clonetech | 632180 |
| EGFP tagged Paxillin hIPSC | Allen Institute | N/A |
| Oligonucleotides | ||
| Scramble gRNA (5’-GCACTACCAGAGCTAACTCA-3’) | This paper | N/A |
| MYH9 gRNA (5’-TCAAGGAGCGTTACTACTCA-3’) | This paper | N/A |
| MYH10 gRNA (5’-TGGATTCCATCAGAACGCCA-3’) | This paper | N/A |
| VCL gRNA (5’-GCGCACGATCGAGAGCATCC-3’) | This paper | N/A |
| MYH6 gRNA (5’-CTGCCGGTGTACAATGCCG-3’) | This paper | N/A |
| MYH7 gRNA (5’-ACTCAGCCGACCTGCTCAA-3’) | This paper | N/A |
| ACTN2C gRNA (5’-ATTTGCCCGCATTATGACCC-3’) | This paper | N/A |
| Recombinant DNA | ||
| plentiCRISPR v2 | Addgene | 52961 |
| pLenti CMV GFP Puro | Addgene | 17448 |
| pEGFP-ACTN2 | Addgene | 52669 |
| pLenti CMV GFP-ACTN2 Puro | This paper | N/A |
| pLenti CMV mApple-ACTN2 Puro | This paper | N/A |
| plentiCRISPR v2 MYH9 gRNA | This paper | N/A |
| plentiCRISPR v2 MYH10 gRNA | This paper | N/A |
| plentiCRISPR v2 ACTN2C gRNA | This paper | N/A |
| plentiCRISPR v2 VCL gRNA | This paper | N/A |
| Software and Algorithms | ||
| Matlab R2015B | MathWorks (https://www.mathworks.com/) | RRID:SCR_001622 |
| Graphpad Prism 6 | http://www.graphpad.com | RRID:SCR_002798 |
| Imaris | BITPLANE (http://www.bitplane.com/imaris/imaris) | RRID:SCR_007370 |
| FIJI (ImageJ) | https://fiji.sc/# | RRID:SCR_002285 |