Fig. 1.
Par3 is asymmetrically localized in the developing OC. Midbasal regions of the OC whole mount stained for Par3 (green). (A–D) At E16.5, Par3 is localized to hair cell-supporting cell contacts and enriched along the lateral borders of hair cells in the control (A and B) but absent in Par3cKO OC (C and D). (E–H) At P0, Par3 distribution at hair cell-supporting cell contacts is no longer planar polarized (E and F). Cell boundaries are labeled by phalloidin staining (F-actin, magenta). Arrowheads indicate the row of pillar cells. Brackets indicate OHC rows. (Scale bars: 6 µm.)