Fig. 8.

Constitutively active Rac1 rescued PCP defects of Par3-deficient cochleae. (A and B) P0 cochlea whole mount stained with phalloidin (magenta). (C) Quantification of cochlear lengths (n = 3 each). (D and E) P0 Rac1^G12V/+ (D) and Par3^cKO; Rac1^G12V/+ OC (E) stained for acetylated tubulin (green) and F-actin (magenta). (F) Quantification of hair bundle orientation. Three embryos of each genotype were scored; n = 603 (Par3^cKO), 293 (Rac1^G12V/+), and 287 (Par3^cKO; Rac1^G12V/+) hair cells. (G) Quantification of kinocilium position index (Methods). Three embryos of each genotype were scored; n = 94 (control), 168 (Par3^cKO), 241 (Rac1^G12V/+), and 226 (Par3^cKO; Rac1^G12V/+) hair cells. (H–M) Hair cell microtubule organization (H and I), centriole (marked by GFP-Centrin2, green) planar polarity (J and K), and asymmetric localization of Vangl2 (L and M) in Rac1^G12V/+ and Par3^cKO; Rac1^G12V/+ OC. (C, F, and G) Error bars represent SD (C) and SEM (F and G). ***P < 0.001. ns, not significant. Lateral side is up in all images. Arrowheads indicate the pillar cell row, and brackets indicate outer hair cell rows. (Scale bars: A and B, 300 µm; and D, E, and H–M, 6 μm.)