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. 2019 Feb 25;116(11):4983–4988. doi: 10.1073/pnas.1819156116

Fig. 7.

Fig. 7.

SHARPIN knockdown affects potential immune/inflammatory functions of megakaryocyte lineage cells. iPS cell-derived immature megakaryocytes were infected with lentivirus expressing either shSHARPIN 1 or shControl shRNA. (A) Following megakaryocyte maturation, surface expression of MHC class I HLA-ABC was determined by flow cytometry (n = 6; mean ± SEM; **P < 0.01). (B) Mature megakaryocytes were incubated for 20 min with vehicle or agonist mixture as in Fig. 4, and sCD40L was determined in cell supernatants by ELISA. Results are normalized for cell count (n = 5; mean ± SEM; *P < 0.05).