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. 2019 Feb 21;116(11):4934–4939. doi: 10.1073/pnas.1820744116

Fig. 3.

Fig. 3.

(A) pH-dependent difference SEIRAS spectra of LacY obtained by subtracting the samples equilibrated at pH 7 minus the samples equilibrated at high pH, inducing a deprotonation of Glu325 at 1,745 cm−1. Pseudo WT and mutants in G46W/G262W mutant background. (B) pH-dependent difference SEIRAS spectra of LacY by subtracting the samples equilibrated at pH 7 minus the samples equilibrated at high pH of the mutations in the LacY mutants in the G46W/G262W mutant background. (C) pH-dependent difference SEIRAS spectra of mutant LacYww/R302K equilibrated at pH 7.0 subtracted from the sample equilibrated at different pH values. ΔAbs, difference in absorption.