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. 2019 Feb 21;116(11):5182–5187. doi: 10.1073/pnas.1815465116

Fig. 3.

Fig. 3.

Expression profiling of GNI1. (A–F) In situ hybridization used to determine the sites of expression of GNI1 during the development of the einkorn wheat spike. (A–D) Longitudinal sections prepared at (A) the floret primordium stage, (B) the terminal spikelet stage, (C) the white anther stage, and (D) the green anther stage. (E) A cross-section made at the white anther stage. (F) Control hybridization using the sense probe. fl, floret; ra, rachilla; sm, spikelet meristem. (Scale bars: 200 µm.) (G–I) Transcription profiling of GNI1 in einkorn wheat and barley (Vrs1) (G), in tetraploid wheat (H), and in bread wheat cv. Bobwhite (I). Values shown as mean ± SE (n = 3). AP, awn primordium stage; FP, floret primordium stage; GA, green anther stage; GP, glume primordium stage; LP, lemma primordium stage; SP, stamen primordium stage; TP, tipping stage; TS, terminal spikelet stage; WA, white anther stage; YA, yellow anther stage.