Fig. 2.

Rev-erbα–mediated neuroinflammation is associated with dysregulated microglial NF-κB signaling. (A) Relative expression of transcripts related to different aspects of neuroinflammation taken from microarray analysis performed on 5-mo RKO and WT mice (n = 3 per genotype). Colored bars on Left indicate hand-curated functional groupings. (B) Gene Ontology (GO) term analysis of microarray data for biological processes up-regulated in RKO hippocampus compared with WT (P > 0.05, fold change >2). (C) Representative images showing p65 nuclear translocation in RKO microglia compared with WT at baseline, 1 h and 3 h, following LPS stimulation. Quantification of the percent microglia with nuclear p65 staining (DAPI/p65 overlap) is shown (Right). n = 3 separate experiments. (D) qPCR showing mRNA expression for the proinflammatory mediators Il6 and Tnfa in primary WT and RKO microglia. (E) ChIP analysis of WT microglia for the Rev-erbα target Bmal1, NF-κB signaling components Traf2 as well as Nfkbib, a negative control S100a4 and a normalization control Gapdh. *P < 0.05 or **P < 0.01 by two-tailed t test with Welch’s correction. ****P < 0.001 by two-way ANOVA with Tukey’s multiple comparisons test.