Skip to main content
. 2019 Feb 25;116(11):5126–5134. doi: 10.1073/pnas.1814456116

Fig. 5.

Fig. 5.

Glial wrapping of somatodendritic surface promotes dendritic regeneration. (A) Schematic representation of the timeline for the balding injury paradigm. (B) Class IV da neuron (green) of RepoGal4 > CD4-tdTomato control larvae and of larvae expressing RepoGal4 > CD4-tdTomato, dATRX RNAi at 24 h and 48 h after no injury. Glia membranes are shown in magenta. (Scale bar, 20 μm.) (C) Dendrites (green) of control and RepoGal4 > CD4-tdTomato, dATRX RNAi expressing larvae at 24 h and 48 h after injury. Glia membranes are shown in magenta. (Scale bar, 20 μm.) The Bottom row shows the entire regenerated arbor of control and RepoGal4 > CD4-tdTomato, dATRX RNAi expressing larvae in grayscale. (Scale bar, 50 μm.) (D) Total coverage of regenerated dendritic arbor in control (black) and RepoGal4 > dATRX RNAi (red) expressing larvae normalized to hemisegment area 48 h after no injury (n = 9, 13 respectively, t test, P > 0.05) and injury paradigm (n = 7 each, t test, P < 0.001). (E) Sholl analysis depicting the number of intersections made by regenerated dendritic branches as a function of radius is shown for the regenerated dendritic arbor 48 h after injury of control RepoGal4 > w1118 (black) and RepoGal4 > dATRX RNAi (red) expressing larvae (n = 7 per condition). The total area under the curve decreased in RepoGal4 > tdTomato, dATRX RNAi larvae compared with control (n = 10 each, 7704 μm2 to 5288 μm2, P < 0.001).