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. 2019 Feb 26;116(11):4963–4972. doi: 10.1073/pnas.1813586116

Fig. 5.

Fig. 5.

Light still increases exposure of DHpL helices in mutants with altered light-responsive behavior. (Center) Difference between the percentage of deuteration at each amide site after 1 min of exchange in the light and in the dark for inactive V119A (A) and active V115A (B) mutants with ADP mapped onto the EL346 structure. Residues with no data are dark gray. Sky blue boxes highlight representative peptides from DHpL helices α1 (Left) and α2 (Right) shown with uptake plots for inactive V119A (A) and active V115A (B) compared with WT EL346 in the dark and light with ADP. WT, dark (black); WT, light (vermillion); V119A, dark (purple); V119A, light (sky blue); V115A, dark (green); and V115A, light (yellow). Phosphoacceptor H142 and ATP-coordinating R175 are highlighted in vermillion in the peptide sequence.