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. 2019 Feb 25;116(11):5154–5159. doi: 10.1073/pnas.1815746116

Fig. 4.

Fig. 4.

Pax2 positively regulates Ampk in the mRMCs. Forced encoding of mRMCs with shPax2, oePax2, shAmpk, and oeAmpk modified the (A and D) Mcp-1, (B and E) Ampk, and (C and F) Pax2 at mRNA levels under normative status. (G−L) Western blot analysis and quantitation of the band density of (G, J, H, and K) p-Ampkα and (G, J, I, and L) Pax2 for the mRMCs transfected with shPax2, oePax2, shAmpk, oeAmpk, and their controls for 48 h. The changes in Pax2 and Ampk at mRNA and protein levels in the transfected mRMCs treated with PA with or without 14(15)-EET were presented in SI Appendix, Fig. S9. Data represent mean ± SD (n = 3); ns (no significant difference) P ≥ 0.05, 0.01 < *P < 0.05, 0.001 < **P ≤ 0.01, and ****P ≤ 0.0001 were determined by two-tailed t test.