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. 2019 Feb 22;116(11):4940–4945. doi: 10.1073/pnas.1809105116

Fig. 2.

Fig. 2.

1500011k16Rik encodes a novel polypeptide. (A) Confocal images of mCherry-tagged Mtln peptide; mitochondria were stained by MitoTracker Green FM and nuclei by Hoechst 33342. (Scale bar, 10 μm.) (B) Immunoblotting of isolated mitochondria from NIH 3T3 and cell lysates from NS0 cells for endogenous Mtln. Tom20 and β-actin were used as a loading control. ∆Mtln -1, -2, -3 are different knockouts for the NIH 3T3 cell line. (C) Immunoblotting of mouse organ lysates for endogenous Mtln. PARK7 was used as a loading control (67).