Fig. 3.

Involvement of RNase L in AZA-induced apoptosis. (A–D) Caspase-3/7 activity in (A) WT, (B) RNase L KO, (C) MAVS KO, and (D) RNase L-MAVS DKO A549 cells mock-treated or treated with 50 µM AZA in the absence or presence of 50 μM ZVAD-FMK (a pan-caspase inhibitor). The data are the averages ± SD of three identically treated replicates. Two biological replicates were performed. (E) Western analyses of WT and RNase L KO A549 cells treated with 50 µM AZA or mock-treated for 48 h. The blots were probed with antibody specific for cleaved PARP1 and β-actin antibody. AU, arbitrary unit.