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. 2019 Mar 15;2(2):e201900367. doi: 10.26508/lsa.201900367

Figure 2. Targeted integration of a TCR into the TRAC locus redirects T-cell specificity.

Figure 2.

(A) Cytotoxic lysis of firefly luciferase (fluc)–expressing ML2 cells expressing HLA-B7 or HLA-B15 by KI-TRAC-TCR cells or unedited T cells at indicated T cell to tumor cell ratios after 24 h of co-incubation assessed by luminescence (n = 3 technical replicates of 1 donor). (B) fluc cytotoxicity assay comparing KI-TRAC-TCR cells, RV-TCR, RV-TCR TCRendo−, and mock cells after 24 h of co-incubation with ML2-B7 fluc at indicated T cell to tumor ratios. T cell to tumor ratios were calculated based on the portion of TCRmu+ cells, and equal numbers of TCRmu+ cells of each condition were used for co-incubation (n = 3 technical replicates of two donors). (C) IFN-γ ELISA of the supernatant.