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. 2019 Mar 18;20(Suppl 1):31. doi: 10.1186/s12863-019-0726-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — dCNDP2 is present in both the cytoplasm and the nucleus. a Western blot showing subcellular fractionation of S2 cells. b Western blot showing ectopic expression of eGFP-tagged proteins in salivary glands using the AB1-GAL4 driver. L3, third instar larval stage. c Localization of eGFP-tagged proteins in L3 salivary gland cells. GFP.nls and mCD8:GFP were used as controls for nuclear localization, and cytoplasmic and transmembrane localization, respectively. Scale bar, 20 μm. d dCNDP2 binds multiple sites on polytene chromosomes. The following exposure times were used to acquire images of chromosomes immunostained with α-dCNDP2 antibodies: 800 msec, ∆dCNDP2 and ∆dCNDP2^(rescue); 500 msec, AB1-GAL4 > UAS-dCNDP2. Scale bar, 20 μm