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. 2019 Mar 18;20:11. doi: 10.1186/s12868-019-0494-8

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Pretreatment with the CRF antagonist effectively increased efficacy of CARTp-induced feeding suppression. a Effects over time of a subthreshold dose, and an effective dose of CARTp, αCRF, or CARTp after αCRF pretreatment, on ad libitum solid food intake over 22 h. Results at each separate time point are shown in panels b–d; b food intake effects of 0.5 or 1 µg CARTp, 10 nmol αCRF, αCRF and respective CARTp-dose, or vehicle after 2 h, c 4 h and d 22 h (NS not significant; *p < 0.05; **p < 0.01; ***p < 0.001)