Table 2.

Summary of gene targeting efficiency

Microinjected sgRNAs	sgRNA 2,4,5	sgRNA 1,3,6	sgRNA 1,2,3,4	Total
No. of embryos collected	113	137	179	429
No. of embryos injected	96	109	140	345
No. of recipients	5	5	6	16
No. of gestations	3	3	4	10
No. of pups born	10	3	5	18
No. of live pups	4	2	1	7
No. of mutant pups	3	2	1	6
No. of pups with large fragment deletion	1	0	1	2
Rate of pregnancy	60.0%(3/5)	60.0%(3/5)	66.7%(4/6)	62.5%(10/16)
Rate of mutations	75.0%(3/4)	100.0%(2/2)	100.0%(1/1)	85.7%(6/7)