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. 2019 Mar 3;2019:6587570. doi: 10.1155/2019/6587570

Figure 2.

Figure 2

Detection of CIK cell proliferation, viability, and cytotoxicity. (a) CIK cells were induced with DCs loaded with lysate from RFP-overexpressing (OE-RFP), TXNDC5-overexpressing (OE-TXNDC5), or PADI4-overexpressing (OE-PADI4) ECA-109 cells. The number of CIK cells was counted following trypan blue staining. (b) CIK cell viability was analyzed following trypan blue staining. (c) A CCK8 kit was used to determine the number of ECA-109 cells following treatment with DC-CIK cells. Asterisks indicate significant differences between CIK cells cocultured with DCs loaded with lysate from PADI4-overexpressing cells and CIK cells cocultured with control DCs. ∗ indicates p < 0.05.