Figure 7.

Alterations in Glo1 activity and AGE levels after incubation with the Glo1 inhibitor BBGC for 72 h. Before adding MG and glycine into the medium, the cells in the BBGC group were pretreated with 4 μM BBGC for 12 h. (a) The activity of Glo1 in HUVECs. The experiment was performed in triplicate and repeated four times. (b) Intracellular AGE levels determined by ELISA. The experiment was performed in triplicate and repeated three times. BBGC: S-bromobenzylglutathione cyclopentyl diester. ^∗∗p < 0.01 compared with the control group. ^#p < 0.05, ^##p < 0.01 compared with cells treated with 400 μM MG.