Table 2.
Fat content in tissues of C57BL/6J (B6) and B6.A-(rs3676616-D10Utsw1)/KjnB6 (B6.A) mice after 12 weeks of high fat diet (HFD) feeding.
| Males | Females | |||
|---|---|---|---|---|
| B6 | B6.A | B6 | B6.A | |
| Fat pads (g)†† | 5.818 ± 1.839 | 5.526 ± 1.231 | 4.397 ± 1.436 | 3.932 ± 1.290 |
| Skin and subcutaneous fat (g)∗ | 2.614 ± 1.178 | 3.342 ± 1.448 | 2.073 ± 0.670 | 2.933 ± 0.726 |
| Carcass and tail fat (g)†† | 2.559 ± 0.668 | 2.470 ± 0.563 | 1.893 ± 0.651 | 1.832 ± 0.493 |
| Organ fat (g) | 0.311 ± 0.084 | 0.397 ± 0.341 | 0.267 ± 0.157 | 0.222 ± 0.062 |
| Liver fat (g) | 0.182 ± 0.174 | 0.115 ± 0.081 | 0.081 ± 0.030 | 0.082 ± 0.030 |
| TA muscle fat (au)†† | 15.64 ± 7.25 | 15.90 ± 5.59 | 11.39 ± 6.10 | 11.13 ± 3.64 |
Fat content of tissues was assessed using Soxhlet method for fat extractions. Intramuscular fat of the tibialis anterior (TA) muscle was evaluated using staining of the muscle cross sections with Oil Red dye with the subsequent quantification of the staining intensity. The values are means ± SD (n=8 each). B6 vs B6.A strains; ††P < 0.01, effect of sex; ∗P < 0.05, effect of strain.