Table 3.

Fasting levels of plasma lipids and insulin in C57BL/6J (B6) and B6.A-(rs3676616-D10Utsw1)/KjnB6 (B6.A) mice after 12 weeks of high fat diet (HFD) feeding.

	Males		Females
	B6	B6.A	B6	B6.A
Free fatty acids (mM) (n=8)	1.30 ± 0.38	1.69 ± 0.33∗	1.47 ± 0.27	1.48 ± 0.28
Triacylglycerol (mM)† (n=16)	1.08 ± 0.29	1.37 ± 0.49	0.98 ± 0.29	1.04 ± 0.37
Total cholesterol (mM)† (n=8)	3.96 ± 1.43	4.86 ± 0.77	3.93 ± 0.62	3.67 ± 0.82†
HDL cholesterol (mM)∗† (n=8)	0.94 ± 0.14	0.79 ± 0.09	0.78 ± 0.08	0.72 ± 0.12
LDL cholesterol (mM) (n=8)	2.15 ± 1.18	3.54 ± 0.82∗	2.72 ± 0.57	2.29 ± 0.91†
Insulin (μg L-1)††† (n=7)	9.39 ± 3.27	7.19 ± 3.82	2.78 ± 0.54††	2.79 ± 0.30†

Plasma concentrations of free fatty acids (FFA), triacylglycerol (TAG), total cholesterol (TC, mM), and HDL cholesterol (HDL-C) were assessed using commercially available kits. LDL cholesterol (LDL-C) concentration was quantified as suggested by Friedewald et al. [35]. Plasma concentrations of insulin were measured using a standard enzyme-linked immunosorbent assay (insulin ELISA, # nr 10-1247-01, Mercodia, Sweden). The values are means ± SD. ^∗P < 0.05, B6 vs B6.A strain; ^†P < 0.05, ^††P < 0.01, ^†††P < 0.001 males vs females.