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. 2019 Mar 15;11:2179–2188. doi: 10.2147/CMAR.S195360

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© 2019 Li et al. This work is published and licensed by Dove Medical Press Limited

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GNL3 knockdown induces cell cycle arrest and promotes apoptosis in osteosarcoma cells.

Notes: (A, B, C) Flow cytometry analyses were applied to test the effects of si-GNL3 on cell cycle distribution in MG63 and U20S cells. (D, E) Cell apoptosis was also assessed by flow cytometry in MG63 and U20S cells. (F) Hoechst/PI staining assay was used to observe the effect of si-GNL3 on cell apoptosis in MG63 and U20S cells. Magnification ×100. (G) After 48 hours of transfection, Western blot assays of apoptosis-related proteins (cleaved Caspase3 and cleaved Caspase9) were conducted in MG63 and U20S cells. Data are presented as the mean±SD (n=3). Results were obtained in three replicates. *P<0.05, **P<0.01 vs the control group.

Abbreviations: NC, negative control; PI, propidium iodide.