Figure 6.

XBP1 binds to GNL3 in osteosarcoma cells.

Notes: (A) The pGL3.0-GNL3-promoter or pGL3.0 Basic vector was constructed to co-transfect with pcDNA3.1-XBP1 vector into MG63 and U20S cells, and pGL3.0-GNL3-promoter was used as control. After co-transfection, dual-luciferase reporter assay was used to assess the relative luciferase activity in MG63 and U20S cells. The si-GNL3 was co-transfected with pcDNA3.1-XBP1 into MG63 and U20S cells. After co-transfection, Western blot was used to detect the expression of GNL3, N-cadherin, and E-cadherin (B, C, and D). (E, F) CCK8 assay was used to assess the effect of XBP1 on cell viability in MG63 (E) and U20S (F). (G, H) Transwell assay was employed to assess the effect of XBP1 on cell invasion ability of MG63 and U20S cells. Magnification ×100. Data are presented as mean±SD (n=3). Results were obtained in three replicates. *P<0.05, **P<0.01 vs the control group.

Abbreviations: NC, negative control; XBP1, X-box-binding protein 1.