Figure 2.

ABHD5 knockdown suppressed endometrial cancer cell proliferation, invasion and the Warburg effect.

Notes: (A) HEC-1A cells were transduced with ABHD5 shRNA and control shRNA (shNC). Cells without any treatment served as a control. Western blot analysis was performed to evaluate the efficiency of three ABHD5 shRNAs in HEC-1A cells at 48 hours post treatment. Representative blots from three independent experiments are shown. (B) Cell proliferation was determined by CCK-8 assay (n=3 biological replicates) in HEC-1A cells transfected with shABHD5#2 and shNC. (C) A Transwell assay (n=3 biological replicates) was performed to assess cell invasive ability, magnification 200×. (D) 2-NBDG uptake was measured (n=3 biological replicates) at 24 hours post treatment. Fluorescent intensity was normalized to protein content and then divided by the value of the control. (E) Lactate production (n=3 biological replicates) was measured and divided by the value of the control. (F) Western blot analysis was performed to detect the related proteins. Representative blots from three independent experiments are shown. ***P<0.001 vs the control and shNC.

Abbreviations: 2-NBDG, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxyglucose; CCK-8, Cell Counting Kit-8.