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. 2019 Mar 15;12:2035–2046. doi: 10.2147/OTT.S188449

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© 2019 Cheng et al. This work is published and licensed by Dove Medical Press Limited

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The localization and expression of cellular senescence-inhibited gene (CSIG) in hepatocellular carcinoma (HCC) cells.

Notes: (A–C) Subcellular localization of CSIG in HCC cells was examined by immunofluorescence analysis. CSIG protein was immune-labeled with anti-CSIG (Sigma-Aldrich Co.) and nucleus was counterstained with DAPI. (A) SMMC7721, (B) MHCC97H, (C) Huh7. (D) CSIG protein levels in immortalized human hepatic cell line L02, and six HCC cell lines (SMMC7721, Huh7, MHCC97L, MHCC97H, HepG2, and Bel7402) were detected by Western blotting.