Table 1.
Primer sequences used for amplification of the indicated components of the endocannabinoid system within qRT-PCR.
| Gene | Primer sequences* | |
|---|---|---|
| NAPE-PLD | Forward | 5′-AAGAGATAGGAAAAAGATTTGGACCTT-3′ |
| Reverse | 5′-CTGGGTCTACATGCTGGTATTTCA-3′ | |
| FAAH | Forward | 5′-GGAGACCAAACAGAGCCTTGAG-3′ |
| Reverse | 5′-CTGAAGAGCCCACCTGTTGAC-3′ | |
| CB1 | Forward | 5′-TGCTGAACTCCACCGTGAAC-3′ |
| Reverse | 5′-TCCCCCATGCTGTTATCCA-3′ | |
| CB2 | Forward | 5′-GCCCAGCCACCCACAAC-3′ |
| Reverse | 5′-GCTATCTCTGTCACCCAGCATTC-3′ | |
| GPR55 | Forward | 5′-GGAAAGTGGAAAAATACATGTGCTT-3′ |
| Reverse | 5′-AACACCTCCAGCGGGAAGA-3′ | |
| TRPV1 | Forward | 5′-GAAGCCGTTGCTCAGAATAACTG-3′ |
| Reverse | 5′-AGCATGGCTTTCAGCAGACA-3′ |
*
All the primer sequences listed were designed using Primer Express software (Applied Biosystems, Warrington, UK) and purchased as HPLC-purified versions from Sigma-Aldrich (Poole, Dorset, UK).
The primers for the housekeeping genes (β-actin (BACT), tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein ζ (YWHAZ) and splicing factor 3a, subunit 1 (SF3A1), were designed and purchased as part of a SYBR green geNorm kit (Primer Design Ltd., Chandler’s Ford, Southampton, Hampshire, UK).