Table 1.
Validation of the established ribosomal subunit protein (rsp) typing scheme with MALDI-TOF MS measurements of in-house group B Streptococcus (GBS) isolates.
| (A) MALDI-TOF MS measurements of 37 isolates with available whole genome sequence (WGS) data. | ||||
|---|---|---|---|---|
| n isolates | source | MALDI assigned rsp-profile | in silico assigned rsp-profile | n correctly assigned |
| 3 | Human | rsp-profile 2 | rsp-profile 2 | 3 |
| 1 | Human | rsp-profile 3 | rsp-profile 3 | 1 |
| 1 | Human | rsp-profile 4 | rsp-profile 4 | 1 |
| 3 | Human | rsp-profile 5 | rsp-profile 5 | 3 |
| 1 | Human | rsp-profile 6 | rsp-profile 6 | 1 |
| 5 | Camel | rsp-profile 11 | rsp-profile 11 | 5 |
| 3 | Camel | rsp-profile 15 | rsp-profile 15 | 3 |
| 2 | Camel | rsp-profile 18 | rsp-profile 18 | 2 |
| 2 | Bovine | rsp-profile 19 | rsp-profile 19 | 2 |
| 2 | Camel | rsp-profile 20 | rsp-profile 20 | 2 |
| 2 | Camel | rsp-profile 21 | rsp-profile 21 | 2 |
| 1 | Camel | rsp-profile 22 | rsp-profile 22 | 1 |
| 1 | Human | rsp-profile 22 | rsp-profile 22 | 1 |
| 1 | Camel | rsp-profile 37 | rsp-profile 37 | 1 |
| 1 | Human | rsp-profile 6 | rsp-profile 55 | 0 |
| 8 ∗ | Fish | rsp-profile 5 | rsp-profile 5 | 8 |
| 37/37 assigned (100% sensitivity) | specificity: 97% (36/37) | |||
| (B) MALDI-TOF MS measurements of 219 isolates without WGS available. | ||||
| n assigned | source | MALDI assigned rsp-profile | ||
| 33 | Human | rsp-profile 2 | ||
| 16 | Human | rsp-profile 3 | ||
| 49 | Human | rsp-profile 4 | ||
| 22 | Human | rsp-profile 5 | ||
| 30 | Human | rsp-profile 6 | ||
| 1 | Human | rsp-profile 7 | ||
| 22 | Camel | rsp-profile 11 | ||
| 20 | Camel | rsp-profile 15 | ||
| 17 | Camel | rsp-profile 18 | ||
| 2 | Bovine | rsp-profile 19 | ||
| 2 | Camel | rsp-profile 20 | ||
| 1 | Camel | rsp-profile 21 | ||
| 1 | Camel | rsp-profile 37 | ||
| 0 (3)∗∗ | Human | new profile | ||
| 216/219 assigned (99% sensitivity) | ||||
*Isolates typed in an external laboratory on a different MALDI-TOF MS system. ∗∗Three isolates displayed novel rsp-profiles not yet contained in our reference database.