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. 2018 May 9;35(10):1192–1203. doi: 10.1089/neu.2017.5401

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Copyright 2018, Mary Ann Liebert, Inc.

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FIG. 5. — Activated microglia/microphages following under-vehicle blasts and mitigation by incorporation of polyurea coated cylinders. (A) Representative fluorescent images of F4/80 immunopositive cells (green) showing overlap with inducible nitric oxide synthase (iNOS) immunopositive cells (red) and 4′,6-diamidino-2-phenylindole (DAPI) as counterstain (blue). Scale bar is 100 μm. (B) Quantification of the F4/80 immunopositive area in the cortex suggests dramatic increase of activated microglia/microphages in the brains of rats subjected to lethal blast intensity with no polyurea coating compared with sham rats (n = 5, **p < 0.01). Levels of activated microglia/microphages were significantly reduced (n = 6; *p < 0.05) in the brains of rats exposed to blast with polyurea coating mitigation system.