Multimodal profiling of single-cell morphology, electrophysiology and gene expression using Patch-seq

. Author manuscript; available in PMC: 2019 Mar 18.

Published in final edited form as: Nat Protoc. 2017 Nov 16;12(12):2531–2553. doi: 10.1038/nprot.2017.120

Cycle	Temperature (°C)	Time	Purpose
1	72	3 min	Extend
2	95	30 s	Denature
3–14	95	10 s	Denature
	55	30 s	Anneal
	72	30 s	Extend
15	72	5 min	Extend
16	4	Hold	Safe storage