| Component | Volume (μL) | Final Concentration |
|---|---|---|
| Nuclease-free sterile water | 1.75 | – |
| Triton X-100 (0.33% vol/vol) | 1.15 | 0.1% (vol/vol) |
| dNTPs (25 mM each) | 0.80 | 5 mM (each dNTP) |
| Oligo dT30VN (100 μM) | 0.10 | 2.5 μM |
| Recombinant RNase Inhibitor (40 U/μL) | 0.10 | 1 U/μL |
| ERCC spike-ins (1:4×10−4 dilution) | 0.10 | 1.6×10−5 dilution |
| Total Volume | 4.00 | – |
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